Hemoglobin is one of the main elements of blood. This protein is a choice marker for blood detection even when the quantity is very low. Moreover, thanks to the use of monoclonal antibodies, obtained from very complex cell culture techniques, immunology methods, used also in forensic medicine, provide both very high sensitivity and high specificity for human blood detection.
Bluestar Identi-HEM Human Blood Detection Test is a rapid, qualitative immunochromatographic test, in a device, for specific detection of human hemoglobin.
This method uses a unique combination of monoclonal antibodies bound to colloidal gold and polyclonal antibodies bound onto a solid phase in order to selectively identify, with high sensitivity and specificity, human hemoglobin.
After being collected with the provided swab, the sample is placed into an extraction buffer. After the sample has been dispensed on the cassette, the extraction medium is absorbed through migration, the antibodies bound to the colloidal gold then bind to the hemoglobin to form an antibody-antigen complex.
This complex then binds to the anti-hemoglobin antibodies present into the test area, and then a colored line appears. If there is no hemoglobin, no colored line appears in the test area.
The mix keeps on migrating onto the membrane, goes beyond the test area, and then binds to the reagents into the control area to produce a colored line, verifying the reaction performed correctly.
Excellent for use with Bluestar Blood Reagent. After locating suspected blood you can test the area for the presence of human blood.